LSBio
IHC-plus™ KRT18 / CK18 / Cytokeratin 18 Antibody (clone KRT18/2819R)

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

IHC-plus™ KRT18 / CK18 / Cytokeratin 18 Antibody (clone KRT18/2819R)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Recombinant Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all KRT18 / CK18 / Cytokeratin 18 antibodies →
$470.00each
Catalog Number: LS-B16287-50

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Overview

Description
Cytokeratin 18 antibody LS-B16287 is an unconjugated rabbit recombinant monoclonal antibody to human Cytokeratin 18 (KRT18 / CK18). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
FC, IF, IHC, IHC-P, WB
Reactivity
Hu
Nonreactive Species
Chicken, Xenopus
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names
CK-18 | Cytokeratin 18 | Cytokeratin-18 | K18 | Keratin 18 | KRT18 | CYK18 | Keratin-18
Recommended Dilution: FC
0.5 - 1 µg/10E6 cells
Recommended Dilution: IF/ICC
1 - 2 µg/ml
Recommended Dilution: IHC-P
1:100
Recommended Dilution: WB
0.5 - 2 µg/ml
UniProt Number
P05783
NCBI GenBank
NM_000224 | NP_000215.1
SwissProt
P05783

Target

Target
KRT18 / CK18 / Cytokeratin 18
Antigen Species
Human
Specificity
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Epitope
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Gene Name
KRT18

Antibody

Host Species
Rabbit
Clonality
Recombinant Monoclonal
Clone
KRT18/2819R
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant full-length human KRT18 protein. Antigen Molecular Weight: 45 kDa
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Concentration
200 µg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 2 years.
Recommended Storage Buffer
10 mM PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.