Anti-IVL / Involucrin antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ IVL / Involucrin Antibody (clone SY5)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$383.00each

Catalog Number: LS-B6298-50

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Overview

Description: Involucrin antibody LS-B6298 is an unconjugated mouse monoclonal antibody to human Involucrin (IVL). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IHC, IHC-F, IHC-P, IP, WB
Reactivity: Hu
Nonreactive Species: Mouse
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: Involucrin | IVL
Recommended Dilution: IHC-P: 10 µg/ml
UniProt Number: P07476
NCBI GenBank: NM_005547 | NP_005538.2
SwissProt: P07476

Target

Target: IVL / Involucrin
Antigen Species: Human
Specificity: The epitope maps between codon 421-568 of human involucrin
Epitope: Recognizes human involucrin, a 585 amino acid soluble protein encoded by the IVL gene and expressed in epidermal keratinocytes as they tend towards terminal differentiation. Involucrin is produced in the stratum spinosum initially in the cell cytosol from where it moves to the cell membrane, associates with transglutaminase and becomes cross-linked to membrane proteins, offering structural support to the cell and forming a stable envelope below the plasma membrane. Recognizes an epitope between residues 421-568 of human involucrin. Involucrin expression is seen in the normal granular cells of the epidermis associated with the plasma membrane during the early phase of cornified envelope maturation and expression is increased in psoriatic epidermis with abnormal cornified envelope maturation. Involucrin staining is also maintained, associated with the cornified envelope in epidermolytic hyperkeratosis, a condition associated with mutations to mutations in genes coding for cytokeratins 1 and 10.
Gene Name: IVL

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: SY5
Origin Species: Human

Immunogen

Immunogen: Native, from keratinocytes.

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Concentration: 0.5 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS