LSBio
IHC-plus™ HLA-DR Antibody (clone YE2/36-HLK)

Anti-HLA-DR antibody IHC of human thymus, medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ HLA-DR Antibody (clone YE2/36-HLK)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
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$470.00each
Catalog Number: LS-B7229-0.125

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Overview

Description
HLA-DR antibody LS-B7229 is an unconjugated rat monoclonal antibody to human HLA-DR. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
Application
FC, IHC, IHC-F, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: FC
1:50 - 1:100
Recommended Dilution: IHC-P
10 µg/ml

Target

Target
HLA-DR
Antigen Species
Human
Specificity
Recognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB. Reacts with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes
Epitope
Recognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB. Reacts with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
YE2/36-HLK

Immunogen

Immunogen
EHR-B Ramos cells.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.