LSBio
IHC-plus™ Histone H4 Antibody (Acetyl-Lys12)

Anti-Histone H4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ Histone H4 Antibody (Acetyl-Lys12)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Non-Human Primate, Rat
Format:Liquid
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$495.00each
Catalog Number: LS-B4750-50

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Overview

Description
Histone H4 antibody LS-B4750 is an unconjugated rabbit polyclonal antibody to Histone H4 (Acetyl-Lys12) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, NHP, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: IHC-P
1:100
Recommended Dilution: WB
1:1000

Target

Target
Histone H4
Antigen Species
Human
Specificity
Recognizes endogenous levels of human histone H4 acetylated at Lys12. It does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat. Note: Adherence to the attached protocol is extremely important.
Epitope
Recognizes endogenous levels of human histone H4 acetylated at Lys12. It does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat. Note: Adherence to the attached protocol is extremely important.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic acetylpeptide (KLH coupled) corresponding to residues surrounding Lys12 of human histone H4.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Volume
50 Microliter
Animal Free
No
Carrier-Free
No
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
10 mM HEPES, 150 mM NaCl

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