LSBio
IHC-plus™ Histone H3 Antibody (Acetyl-Lys18)

Anti-Histone H3 antibody IHC of human breast epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-7.5 ug/ml.

IHC-plus™ Histone H3 Antibody (Acetyl-Lys18)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B8486-50

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Overview

Description
Histone H3 antibody LS-B8486 is an unconjugated rabbit polyclonal antibody to Histone H3 (Acetyl-Lys18) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:133 - 1:200
Recommended Dilution: WB
1:500 - 1:1000

Target

Target
Histone H3
Antigen Species
Human
Specificity
Histone H3 (Acetyl-Lys18) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18.
Epitope
Histone H3 (Acetyl-Lys18) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic peptide from human Histone H3 around the acetylated site of Lys18. (AA Range: 11-60)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year.
Recommended Storage Buffer
PBS, 150 mM NaCl

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