Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Histone H2B Antibody (aa111-125)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Bovine, Chicken, Fruit Fly, Human, Arthropod (Insect / Arachnid / Crustacean), Mouse, Rat, Zebrafish
Format:	Liquid

$383.00each

Catalog Number: LS-B2035-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Histone H2B antibody LS-B2035 is an unconjugated rabbit polyclonal antibody to Histone H2B (aa111-125) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application: IHC, IHC-P, WB
Reactivity: Bov, Ck, Dros, Hu, Arth, Ms, Rt, Zf
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Histone H2B
Recommended Dilution: IHC-P: 5 µg/ml
Recommended Dilution: WB: 2 µg/ml

Target

Target: Histone H2B
Antigen Species: Human
Specificity: A synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila.
Epitope: A 15 kD band is observed. This is routinely quality controlled in human lysates.

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.05% Sodium Azide, 0.05% BSA
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: No
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: PBS

Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Spiró Z, Koh A, Tay S, See K, Winkler C. Scientific reports. 2016;6:27470. PubMed: 27273160 PMC: PMC4895340

Submit a Publication