Anti-GPR109B / HM74 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ HCAR3 / GPR109B / HM74 Antibody (Cytoplasmic Domain)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general)
Reactivity:	Dog, Hamster, Human
Format:	Liquid

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$403.00each

Catalog Number: LS-A180-50

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Overview

Description: HM74 antibody LS-A180 is an unconjugated rabbit polyclonal antibody to HM74 (HCAR3 / GPR109B) (Cytoplasmic Domain) from human. It is reactive with human, dog and hamster. Validated for ICC and IHC. Tested on 20 paraffin-embedded human tissues.
Application: IHC
Reactivity: Can, Ham, Hu
Predicted Reactivity: Guinea Pig, Horse, Mouse, Rat
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GPR109B | HCAR3 | GTP-binding protein | NIACR2 | HM74 | HM74b | Nicotinic acid receptor 2 | Putative chemokine receptor | Niacin receptor 2 | Puma-g | HCA3 | PUMAG
Recommended Dilution: IHC-P: 19 - 38 µg/ml
UniProt Number: P49019
NCBI GenBank: NM_006018 | NP_006009.2
SwissProt: P49019

Target

Target: HCAR3 / GPR109B / HM74
Antigen Species: Human
Specificity: Human GPR109B / HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100%), GPR81 (50%).
Epitope: Human GPR109B / HM74. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HCAR2 (100%), GPR81 (50%).
Gene Name: HCAR3

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR109B / HM74. Percent identity with other species by BLAST analysis: Human, Gorilla, Dog, Hamster, Panda (100%); Mouse, Horse, Guinea pig (95%); Rat (90%).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.1% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Recommended Storage Buffer: PBS