LSBio
IHC-plus™ GSK3B / GSK3 Beta Antibody (phospho-Ser9)

Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ GSK3B / GSK3 Beta Antibody (phospho-Ser9)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B55-50

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Overview

Description
GSK3 Beta antibody LS-B55 is an unconjugated rabbit polyclonal antibody to human GSK3 Beta (GSK3B) (pSer9). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Dog, Chicken, Other, Rat, Zebrafish
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GSK3B | GSK-3 beta | GSK3 beta | GSK3beta | GSK3beta isoform | TPKI | GSK 3beta | Tau protein kinase I | ZGSK-3beta
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IF/ICC
1:200 - 1:1000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
P49841
SwissProt
P49841

Target

Target
GSK3B / GSK3 Beta
Antigen Species
Human
Specificity
This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, rat, frog, chicken, dog and zebrafish based on 100% homology for the immunogen sequence. Cross reactivity with GSK3B homologues from other sources has not been determined.
Epitope
This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, rat, frog, chicken, dog and zebrafish based on 100% homology for the immunogen sequence. Cross reactivity with GSK3B homologues from other sources has not been determined.
Gene Name
GSK3B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-15 of human GSK3 beta.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Allodi I, Comley L, Nichterwitz S, Nizzardo M, Simone C, Benitez JA, Cao M, Corti S, Hedlund E. Scientific reports. 2016;6:25960. PubMed: 27180807 PMC: PMC4867585

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