LSBio
IHC-plus™ GOK / STIM1 Antibody (C-Terminus)

Anti-GOK / STIM1 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ GOK / STIM1 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B10807-50

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Overview

Description
STIM1 antibody LS-B10807 is an unconjugated rabbit polyclonal antibody to human STIM1 (GOK) (C-Terminus). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Mouse, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
D11S4896E | STIM1 | Stromal interaction molecule 1 | GOK | STIM1L
Recommended Dilution: ELISA
1:10000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 µg/ml
UniProt Number
Q13586
NCBI GenBank
NM_003156 | NP_003147.2
SwissProt
Q13586

Target

Target
GOK / STIM1
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest cross-reactivity with rat, human, bovine, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.
Epitope
A BLAST analysis was used to suggest cross-reactivity with rat, human, bovine, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.
Gene Name
STIM1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Stim 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human Stim 1.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 1 mg/ml BSA, 50% Glycerol
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
STIM1 R304W causes muscle degeneration and impaired platelet activation in mice. Gamage TH, Gunnes G, Lee RH, Louch WE, Holmgren A, Bruton JD, Lengle E, Kolstad TRS, Revold T, Amundsen SS, Dalen KT, Holme PA, Tjønnfjord GE, Christensen G, Westerblad H, Klungland A, Bergmeier W, Misceo D, Frengen E. Cell calcium. 2018 December;76:87-100. PubMed: 30390422 PMC: PMC6481308
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