LSBio
IHC-plus™ GNAI1 / Gi Antibody (clone R4.5)

Anti-GNAI1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHC-plus™ GNAI1 / Gi Antibody (clone R4.5)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Guinea Pig, Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B2546-50

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Overview

Description
Gi antibody LS-B2546 is an unconjugated mouse monoclonal antibody to Gi (GNAI1) from rat. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Bov, GP, Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Gi1 protein alpha subunit | Gi | GNAI1
Recommended Dilution: IHC-P
1:50
UniProt Number
P63096
SwissProt
P63096

Target

Target
GNAI1 / Gi
Antigen Species
Rat
Specificity
Recognizes Rat Gai1. Does not cross-react with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species cross-reactivity: mouse, bovine, guinea pig and human.
Epitope
Recognizes Rat Gai1. Does not cross-react with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species cross-reactivity: mouse, bovine, guinea pig and human.
Gene Name
GNAI1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
R4.5
Origin Species
Human

Immunogen

Immunogen
Partially purified Gai1 protein from rat brain (39-42kD). Cellular Localization: Cell membrane.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Concentration
0.2 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Heterotrimeric G-protein, Gi1, is involved in the regulation of proliferation, neuronal migration, and dendrite morphology during cortical development in vivo. Nanako Hamada, Ikuko Iwamoto, Noriko Kawamura, Koh-Ichi Nagata. Journal of neurochemistry. 2021 May;157:1167-1181. PubMed: 33025585

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