LSBio
IHC-plus™ GLI3 Antibody

Anti-GLI3 antibody IHC of human brain-glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.25 ug/ml.

IHC-plus™ GLI3 Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
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$526.00each
Catalog Number: LS-B73-50

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Overview

Description
GLI3 antibody LS-B73 is an unconjugated rabbit polyclonal antibody to GLI3 from human. It is reactive with human, chicken, chimpanzee and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, NHP
Predicted Reactivity
Bird / Avian (Other), Dog, Chicken, Non-Human Primate, Xenopus
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ACLS | GCPS | GLI-Kruppel family member GLI3 | GLI3 full length protein | GLI3-190 | GLI family zinc finger 3 | GLI3 | Gli-3 | GLI3FL | PAP-A | PAPA1 | PHS | PPDIV | Transcriptional activator GLI3 | PAPA | PAPB | GLI3 form of 190 kDa | Oncogene GLI3 | Zinc finger protein GLI3
Recommended Dilution: ELISA
1:6000 - 1:30000
Recommended Dilution: IHC-P
1.25 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P10071
NCBI GenBank
NM_000168 | NP_000159.3
SwissProt
P10071

Target

Target
GLI3
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.
Epitope
A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.
Gene Name
GLI3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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