GJB2 / CX26 / Connexin 26 Antibody
Catalog Number: LS-C664701-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Anti-GJB2 antibody IHC of rat intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ GJB2 / CX26 / Connexin 26 Antibody (Cytoplasmic Domain, clone CX-12H10)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$495.00each
Catalog Number: LS-B5511-50
Catalog Number: LS-B5511
For bulk orders or custom formulations:
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- Description
- Connexin 26 antibody LS-B5511 is an unconjugated mouse monoclonal antibody to Connexin 26 (GJB2 / CX26) (Cytoplasmic Domain) from rat. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-F, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Connexin 26 | Connexin-26 | DFNB1A | DFNA3 | DFNB1 | Gap junction protein beta 2 | GJB2 | KID | HID | PPK | NSRD1 | CX26 | DFNA3A
- Recommended Dilution: ELISA
- 0.1 - 1 µg/ml
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P29033
- NCBI GenBank
- NM_004004 | NP_003995.2
- SwissProt
- P29033
Target
- Target
- GJB2 / CX26 / Connexin 26
- Antigen Species
- Rat
- Specificity
- Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.
- Epitope
- Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.
- Gene Name
- GJB2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- CX-12H10
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide
- Concentration
- 0.5 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Mammary gland specific knockdown of the physiological surge in Cx26 during lactation retains normal mammary gland development and function. Stewart MK, Plante I, Bechberger JF, Naus CC, Laird DW. PloS one. 2014;9:e101546. PubMed: 24988191 PMC: PMC4079510
