LSBio
IHC-plus™ GFAP Antibody (clone GA-5)

Anti-GFAP antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ GFAP Antibody (clone GA-5)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Pig, Rat
Format:Liquid
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$470.00each
Catalog Number: LS-B4512-0.05

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Overview

Description
GFAP antibody LS-B4512 is an unconjugated mouse monoclonal antibody to GFAP from human. It is reactive with human, rat and porcine. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ICC, IHC, IHC-P, IP, WB
Reactivity
Hu, Por, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
GFAP
Recommended Dilution: IF/ICC
1 - 4 µg/ml
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1 - 2 µg/ml
UniProt Number
P14136
NCBI GenBank
NM_002055 | NP_002046.1
SwissProt
P14136

Target

Target
GFAP
Antigen Species
Human
Specificity
The antibody GA-5 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Epitope
The antibody GA-5 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Gene Name
GFAP

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
GA-5
Origin Species
Human

Immunogen

Immunogen
Porcine spinal cord.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 15 mM Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze.
Recommended Storage Buffer
PBS

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