Anti-GAD65 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ GAD65 Antibody (aa4-22, clone N-GAD65)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot
Reactivity:	Human
Format:	Liquid

$383.00each

Catalog Number: LS-B9984-50

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Overview

Description: GAD65 antibody LS-B9984 is an unconjugated mouse monoclonal antibody to human GAD65 (aa4-22). Validated for IHC, IP, RIA and WB. Tested on 20 paraffin-embedded human tissues.
Application: IHC, IHC-P, IP, RIA, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: GAD-65 | GAD2 | Glutamate decarboxylase 2 | GAD65
Recommended Dilution: IHC-P: 5 µg/ml
Recommended Dilution: WB: 1:100 - 1:1000
UniProt Number: Q05329
NCBI GenBank: NM_000818 | NP_000809.1
SwissProt: Q05329

Target

Target: GAD65
Antigen Species: Human
Specificity: Specifically recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2, otherwise known as GAD65/GAD2, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, principally expressed in the brain and also in pancreatic beta cells. GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67.
Epitope: Recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2, otherwise known as GAD65/GAD2, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, principally expressed in the brain and also in pancreatic beta cells. GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67.
Gene Name: GAD2

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: N-GAD65
Origin Species: Human

Immunogen

Immunogen: Keyhole Limpet Hemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-Terminus of human GAD65. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%); Gibbon, Galago, Rat, Ferret, Panda, Bovine, Cat, Dog, Horse, Pig (95%); Monkey, Marmoset, Mouse, Rabbit, Opossum, Guinea pig (89%).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS