Anti-FOXO3A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ FOXO3 / FOXO3A Antibody (phospho-Thr24/Thr32)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$495.00each

Catalog Number: LS-B3303-50

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Quantity

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Overview

Description: FOXO3A antibody LS-B3303 is an unconjugated rabbit polyclonal antibody to FOXO3A (FOXO3) (pThr24/Thr32) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IHC, IHC-P, IP, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: AF6q21 | AF6q21 protein | Forkhead box O3 | Forkhead box O3A | FOXO3A | FKHRL1P2 | Forkhead box protein O3 | FOXO2 | FKHRL1
Recommended Dilution: IHC-P: 1:100
Recommended Dilution: IP: 1:50
Recommended Dilution: WB: 1:1000
UniProt Number: O43524
NCBI GenBank: NM_001455 | NP_001446.1
SwissProt: O43524

Target

Target: FOXO3 / FOXO3A
Antigen Species: Human
Specificity: Detects endogenous levels of FKHR (FKHRL1) only when activated by phosphorylation at Thr24 of FKHR or Thr32 of FKHRL1. Also reacts with phosphorylated AFX at Thr28. Does not react with FKHR family members phosphorylated at other sites. Species cross-reactivity: Human, mouse, rat.
Epitope: Detects endogenous levels of FKHR (FKHRL1) only when activated by phosphorylation at Thr24 of FKHR or Thr32 of FKHRL1. Also reacts with phosphorylated AFX at Thr28. Does not react with FKHR family members phosphorylated at other sites. Species cross-reactivity: Human, mouse, rat.
Gene Name: FOXO3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr24 of human FKHR.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Volume: 50 Microliter
Animal Free: No
Carrier-Free: No
Preservative: No
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 10 mM HEPES, 150 mM NaCl