LSBio
IHC-plus™ Filamin Antibody (N-Terminus)

Anti-Filamin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

IHC-plus™ Filamin Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Chicken, Guinea Pig, Human, Mouse, Rabbit, Rat
Format:Liquid
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$495.00each
Catalog Number: LS-B6314-50

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Overview

Description
Filamin antibody LS-B6314 is an unconjugated mouse monoclonal antibody to Filamin (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
IF, IHC, IHC-P, IP, WB
Reactivity
Ck, GP, Hu, Ms, Rb, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IF/ICC
1:50 - 1:200
Recommended Dilution: IHC-P
1:50
Recommended Dilution: WB
1:500 - 1:2000

Target

Target
Filamin
Antigen Species
Human
Specificity
Recognizes native, full-length human filamin (actin-binding protein) at 270-280kD and 190kD N-terminal calpain cleavage fragment of filamin. Following induction of apoptosis in U937 cells, recognizes 170, 150, and 120kD N-terminal cleavage fragments of the full-length from presumably resulting from cleavage by activated caspase-3. Species cross-reactivity: Chicken, guinea pig, rabbit and rat.
Epitope
Recognizes native, full-length human filamin (actin-binding protein) at 270-280kD and 190kD N-terminal calpain cleavage fragment of filamin. Following induction of apoptosis in U937 cells, recognizes 170, 150, and 120kD N-terminal cleavage fragments of the full-length from presumably resulting from cleavage by activated caspase-3. Species cross-reactivity: Chicken, guinea pig, rabbit and rat.

Antibody

Host Species
Mouse
Clonality
Monoclonal

Immunogen

Immunogen
Human platelet protein.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.09% Sodium Azide
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Heterozygous Arrhythmogenic Cardiomyopathy- desmoplakin Mutation Carriers Exhibit a Subclinical Cutaneous Phenotype with Cell Membrane Disruption and Lack of Intercellular Adhesion. Eva Cabrera-Borrego , Trinidad Montero-Vilchez , Francisco José Bermúdez-Jiménez , Jesús Tercedor-Sánchez , Luis Tercedor-Sánchez , Manuel Sánchez-Díaz , Rosa Macías-Ruiz , María Molina-Jiménez , Francisco Javier Cañizares-García, Eduardo Fernández-Segura, Angel Fernandez-Flores, Salvador Arias-Santiago , Juan Jiménez-Jáimez. Journal of Clinical Medicine. 2021 Oct;10:4608. PubMed: 34640625 PMC: PMC8509745

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