ESET / SETDB1 Antibody
Catalog Number: LS-C482531
4 sizes / from $272.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-SETDB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ ESET / SETDB1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B720-50
Catalog Number: LS-B720
For bulk orders or custom formulations:
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- Description
- SETDB1 antibody LS-B720 is an unconjugated rabbit polyclonal antibody to human SETDB1 (ESET). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- H3-K9-HMTase4 | ESET | KMT1E | H3-K9-HMTase 4 | KG1T | SET domain, bifurcated 1 | SET domain bifurcated 1 | KIAA0067 | Lysine N-methyltransferase 1E | SETDB1
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IHC-P
- 5 µg/ml
- Recommended Dilution: WB
- 1:1000 - 1:2500
- UniProt Number
- Q15047
- NCBI GenBank
- NM_012432 | NP_036564.3
- SwissProt
- Q15047
Target
- Target
- ESET / SETDB1
- Antigen Species
- Human
- Gene Name
- SETDB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human ESET.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.2 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids. Harte PJ, Wu W, Carrasquillo MM, Matera AG. Cytogenetics and cell genetics. 1999;84:83-6.
PubMed: 10343109
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ. Genes & development. 2002;16:919-32.
PubMed: 11959841 · PMC: PMC152359
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y. Molecular cell. 2003;12:475-87.
PubMed: 14536086
Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Sarraf SA, Stancheva I. Molecular cell. 2004;15:595-605.
PubMed: 15327775
