LSBio
IHC-plus™ EMA / MUC1 Antibody (clone M4H2)

Anti-EMA / MUC1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ EMA / MUC1 Antibody (clone M4H2)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
See all EMA / MUC1 antibodies →
$638.00each
Catalog Number: LS-B8757-0.25

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Overview

Description
MUC1 antibody LS-B8757 is an unconjugated mouse monoclonal antibody to human MUC1 (EMA). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-F, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CA15-3 | CD227 | Carcinoma-associated mucin | CD227 antigen | DF3 antigen | Episialin | Epithelial membrane antigen | H23 antigen | H23AG | Krebs von den Lungen-6 | MAM6 | MUC1/ZD | MUC-1/SEC | Mucin-1 | Pem | KL-6 | MUC-1/X | MUC1 | Tumor-associated mucin | Peanut-reactive urinary mucin | Polymorphic epithelial mucin | PUM | EMA | MUC-1 | Mucin 1, transmembrane
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P15941
SwissProt
P15941

Target

Target
EMA / MUC1
Antigen Species
Human
Specificity
Binds with high efficiency to an epitope within the VNTR recombinant repeat peptide region of MUC1 molecule. Reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (but not with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1, but not with completely glycosylated MUC1. Belongs to MAb clusters 6 and 7 according to ISOMB TD-4 classification. Non cross-reactive with normal serum proteins and human tissues of nonepithelial origin.
Epitope
Binds with high efficiency to an epitope within the VNTR recombinant repeat peptide region of MUC1 molecule. Reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (but not with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1, but not with completely glycosylated MUC1. Belongs to MAb clusters 6 and 7 according to ISOMB TD-4 classification. Non cross-reactive with normal serum proteins and human tissues of nonepithelial origin.
Gene Name
MUC1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
M4H2
Origin Species
Human

Immunogen

Immunogen
High molecular weight (>300kD) glycoprotein from human milk-fat globule molecule.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide
Concentration
8.2 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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