Anti-EIF3F antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ EIF3S5 / EIF3F Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Format:	Liquid

$526.00each

Catalog Number: LS-B366-50

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Quantity

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Overview

Description: EIF3F antibody LS-B366 is an unconjugated rabbit polyclonal antibody to EIF3F (EIF3S5) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IHC, IHC-P, WB
Predicted Reactivity: Bovine, Chicken, Human, Mouse, Non-Human Primate, Rat
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Deubiquitinating enzyme eIF3f | EIF-3-epsilon | EIF3 p47 | EIF3S5 | EIF3-epsilon | EIF3-p47 | EIF3F
Recommended Dilution: ELISA: 1:10000 - 1:40000
Recommended Dilution: IHC-P: 2.5 µg/ml
Recommended Dilution: WB: 1:200 - 1:2000
UniProt Number: O00303
NCBI GenBank: NM_003754 | NP_003745.1
SwissProt: O00303

Target

Target: EIF3S5 / EIF3F
Antigen Species: Human
Specificity: A BLAST analysis was used to suggest cross reactivity with eIF3f protein from human, mouse, rat, chimpanzee, chicken, and bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against eIF3f from Drosophila melanogaster and Tetraodon nigroviridis (pufferfish) based on 90% homology of the immunizing sequence with eIF3f from these sources. Reactivity against homologues from other sources is not known.
Epitope: A BLAST analysis was used to suggest cross reactivity with eIF3f protein from human, mouse, rat, chimpanzee, chicken, and bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against eIF3f from Drosophila melanogaster and Tetraodon nigroviridis (pufferfish) based on 90% homology of the immunizing sequence with eIF3f from these sources. Reactivity against homologues from other sources is not known.
Gene Name: EIF3F

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human eIF3f protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl