Anti-E2F1 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ E2F1 Antibody (phospho-Ser364)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$526.00each

Catalog Number: LS-B52-50

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Overview

Description: E2F1 antibody LS-B52 is an unconjugated rabbit polyclonal antibody to E2F1 (pSer364) from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ELISA, IHC, IHC-P, WB
Reactivity: Hu
Predicted Reactivity: Non-Human Primate
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: E2F transcription factor 1 | E2F1 | PRB-binding protein E2F-1 | RBAP-1 | RBBP3 | RBBP-3 | Transcription factor E2F1 | RBAP1 | E2F-1 | PBR3
Recommended Dilution: ELISA: 1:20000 - 1:100000
Recommended Dilution: IHC-P: 10 µg/ml
Recommended Dilution: WB: 1:250 - 1:2000
UniProt Number: Q01094
NCBI GenBank: NM_005225 | NP_005216.1
SwissProt: Q01094

Target

Target: E2F1
Antigen Species: Human
Specificity: Reactivity occurs against human E2F-1 pS364 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with E2F-1 homologues from other sources has not been determined.
Epitope: Reactivity occurs against human E2F-1 pS364 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with E2F-1 homologues from other sources has not been determined.
Gene Name: E2F1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human E2F-1.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified