LSBio
IHC-plus™ CUL3 / Cullin 3 Antibody (aa1-25)

Anti-CUL3 / Cullin 3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC-plus™ CUL3 / Cullin 3 Antibody (aa1-25)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse
Format:Liquid
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$526.00each
Catalog Number: LS-B359-50

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Overview

Description
Cullin 3 antibody LS-B359 is an unconjugated rabbit polyclonal antibody to Cullin 3 (CUL3) (aa1-25) from human. It is reactive with human and mouse. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Cullin 3 | CUL3 | Cullin homolog 3 | Cullin-3 | KIAA0617 | PHA2E | CUL-3
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC-P
1:500
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
Q13618
NCBI GenBank
NP_003581.1
SwissProt
Q13618

Target

Target
CUL3 / Cullin 3
Antigen Species
Human
Specificity
Reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequence homology. Cross reactivity with other human cullins is unlikely based on sequence homology.
Epitope
Reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequence homology. Cross reactivity with other human cullins is unlikely based on sequence homology.
Gene Name
CUL3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Cul3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal near amino acids 1-25 of Human Cul3 coupled to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.01% Sodium Azide
Concentration
85 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Kipreos ET, Lander LE, Wing JP, He WW, Hedgecock EM. Cell. 1996;85:829-39.

PubMed: 8681378


Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. Furukawa M, He YJ, Borchers C, Xiong Y. Nature cell biology. 2003;5:1001-7.

PubMed: 14528312


New insights into the function of Cullin 3 in trophoblast invasion and migration. Zhang Q, Yu S, Huang X, Tan Y, Zhu C, Wang YL, Wang H, Lin HY, Fu J, Wang H. Reproduction (Cambridge, England). 2015;150:139-49.

PubMed: 26021998

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