LSBio
IHC-plus™ Complement C4c Antibody (clone 10-12)

Anti-Complement C4c antibody IHC staining of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ Complement C4c Antibody (clone 10-12)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$516.00each
Catalog Number: LS-B8738-50

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Overview

Description
Complement C4c antibody LS-B8738 is an unconjugated mouse monoclonal antibody to human Complement C4c. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, FC, IHC, IHC-F, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IHC-P
10 µg/ml

Target

Target
Complement C4c
Antigen Species
Human
Specificity
Recognizes complement component 4c (C4c). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kD) and alpha 3 (25 kD) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma. Binds both C4c and C4 and so is not suitable for all ELISA applications.
Epitope
Recognizes complement component 4c (C4c). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kD) and alpha 3 (25 kD) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma. Binds both C4c and C4 and so is not suitable for all ELISA applications.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
10-12

Immunogen

Immunogen
Purified human C4c.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
BBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
bbs

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.