LSBio
IHC-plus™ Complement C3c Antibody

Anti-Complement C3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ Complement C3c Antibody

Primary AntibodyLSBio Guarantee
Antibody:Sheep Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B5757-50

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Overview

Description
Complement C3c antibody LS-B5757 is an unconjugated sheep polyclonal antibody to human Complement C3c. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-F, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:400
Recommended Dilution: IHC-F
1:50
Recommended Dilution: IHC-P
5 µg/ml

Target

Target
Complement C3c
Antigen Species
Human
Specificity
This antibody detects human Complement 3c, a polypeptide fragment which is present in C3.
Epitope
Detects human complement 3c, a polypeptide fragment which is present in C3. C3 plays a central role in the activation of the complement system. Processing of C3 by C3 convertase is the central reaction in both classical and alternative complement pathways, resulting in C3a and C3b. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates, and help to initiate the complement cascade, potentially resulting in cellular apoptosis. C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c and C3dg. Defects in C3 can result in susceptibility to pyogenic infection. Shows minimal reactivity with related serum proteins.

Antibody

Host Species
Sheep
Clonality
Polyclonal

Immunogen

Immunogen
Native human C3 / Complement C3.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
5 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.