LSBio
IHC-plus™ CENPU / MLF1IP Antibody

Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ CENPU / MLF1IP Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B211-50

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Overview

Description
MLF1IP antibody LS-B211 is an unconjugated rabbit polyclonal antibody to MLF1IP (CENPU) from human. It is reactive with human, bovine, chimpanzee and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Dog, Mouse, Non-Human Primate, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CENP-U | CENP50 | CENPU50 | Centromere protein U | ICEN24 | KLIP1 | Polo-box-interacting protein 1 | MLF1-interacting protein | CENP-50 | CENPU | Centromere protein of 50 kDa | MLF1 interacting protein | MLF1IP | PBIP1
Recommended Dilution: ELISA
1:5000 - 1:25000
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q71F23
SwissProt
Q71F23

Target

Target
CENPU / MLF1IP
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from rat and mouse (90% homology). Reactivity against homologues from other sources is not known. This antibody reacts with MLF1IP protein that is either phosphorylated or non-phosphorylated at Thr78.
Epitope
A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, dog, bovine and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from rat and mouse (90% homology). Reactivity against homologues from other sources is not known. This antibody reacts with MLF1IP protein that is either phosphorylated or non-phosphorylated at Thr78.
Gene Name
CENPU

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.06 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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