LSBio
IHC-plus™ APOE / Apolipoprotein E Antibody

Anti-Apolipoprotein E / APOE antibody IHC of human kidney, glomeruli. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

IHC-plus™ APOE / Apolipoprotein E Antibody

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Rat
Format:Liquid
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$556.00each
Catalog Number: LS-B7857-100

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Overview

Description
Apolipoprotein E antibody LS-B7857 is an unconjugated goat polyclonal antibody to Apolipoprotein E (APOE) from human. It is reactive with human and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AD2 | Apo-E | APOE | Apolipoprotein E3 | Apolipoprotein E | LDLCQ5 | LPG
Recommended Dilution: ELISA
1:4000 - 1:8000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P02649
SwissProt
P02649

Target

Target
APOE / Apolipoprotein E
Antigen Species
Human
Specificity
Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Epitope
Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Gene Name
APOE

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.125 M Sodium Borate, pH 8.0, 0.005 M EDTA, 0.075 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
0.125 M Sodium Borate, 0.075 M NaCl

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