Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ AKT1 + AKT2 + AKT3 Antibody (aa460-480)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Chicken, Human, Mouse, Rat
Format:	Liquid

$526.00each

Catalog Number: LS-B2844-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: AKT1 + AKT2 + AKT3 antibody LS-B2844 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 (aa460-480) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application: ELISA, FC, ICC, IF, IHC, IHC-P, WB
Reactivity: Ck, Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:2000 - 1:10000
Recommended Dilution: IF/ICC: 1:100 - 1:1000
Recommended Dilution: IHC-P: 1:100
Recommended Dilution: WB: 1:500 - 1:2000

Target

Target: AKT1 + AKT2 + AKT3
Antigen Species: Human
Specificity: Reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
Epitope: Reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen: AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 85 mg/mL
Volume: 50 Microliter
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Other

Storage & Handling

Storage Conditions: Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl

Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. Matsuda N, Takano Y, Kageyama S, Hatakeyama N, Shakunaga K, Kitajima I, Yamazaki M, Hattori Y. Cardiovascular research. 2007;76:132-40. PubMed: 17601517

Submit a Publication