Anti-ACTN2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ ACTN2 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$470.00each

Catalog Number: LS-B4852-50

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Overview

Description: ACTN2 antibody LS-B4852 is an unconjugated rabbit polyclonal antibody to human ACTN2. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ICC, IF, IHC, IHC-P, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Actinin, alpha 2 | ACTN2 | Alpha-actinin skeletal muscle | CMD1AA | F-actin cross-linking protein | Alpha-actinin-2
Recommended Dilution: IF/ICC: 1:100 - 1:200
Recommended Dilution: IHC-P: 10 µg/ml
Recommended Dilution: WB: 1:500 - 1:3000
UniProt Number: P35609
NCBI GenBank: NM_001103 | NP_001094.1
SwissProt: P35609

Target

Target: ACTN2
Antigen Species: Human
Specificity: Human alpha Actinin 2. Predicted cross-reactivity based on amino acid sequence homology: mouse (100%), rat (85%), bovine (84%), monkey (100%), zebrafish (86%).
Epitope: Human alpha Actinin 2. Predicted cross-reactivity based on amino acid sequence homology: mouse (100%), rat (85%), bovine (84%), monkey (100%), zebrafish (86%).
Gene Name: ACTN2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
Immunogen Type: Proprietary / Undisclosed

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.1 M Tris, pH 7, 0.1 M Glycine, 10% Glycerol, 0.01% Thimerosal
Concentration: 1 mg/mL
Volume: 50 Microliter
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Keep as concentrated solution. For short term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Recommended Storage Buffer: 0.1 M Tris