Immunofluorescence staining of IER5 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human IER5 polyclonal antibody (dilution ratio 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

IER5 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence
Reactivity:	Human
Format:	Liquid

$309.00each

Catalog Number: LS-C816547-100

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Quantity

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Overview

Description: IER5 antibody LS-C816547 is an unconjugated rabbit polyclonal antibody to human IER5. Validated for ICC and IF.
Application: ICC, IF
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Immediate early response 5 | IER5 | SBBI48
Recommended Dilution: IF/ICC: 1:50 - 1:200
UniProt Number: Q5VY09
NCBI GenBank: NM_016545 | NP_057629.2
SwissProt: Q5VY09

Target

Target: Human IER5
Antigen Species: Human
Epitope: Human IER5
Gene Name: IER5

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: E. coli-derived Human IER5 fragment
Immunogen Type: Recombinant Protein Fragment

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.0, 0.03% Proclin 300
Volume: 100 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS