Western Blot of Anti-IdU Antibody. Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: IdU. Lane 5 CldU. Load: 20 ug per lane. Primary antibody: Anti-IdU antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 alpha mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed: IdU. Other band(s): no cross reactive bands were observed for other nucleoside analogs.
IdU / Iododeoxyuridine Antibody (clone 32D8.D9)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C209694-100
Catalog Number: LS-C209694
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- Description
- Iododeoxyuridine antibody LS-C209694 is an unconjugated mouse monoclonal antibody to Iododeoxyuridine (IdU). Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC, WB
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000 - 1:10000
- Recommended Dilution: WB
- 1:2000 - 1:5000
Target
- Target
- IdU / Iododeoxyuridine
- Epitope
- This antibody is specific for IdU. Cross-reactivity is not observed with BrdU, CldU or FdU.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 32D8.D9
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-IdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with Bromodeoxyuridine-KLH followed by hybridoma development.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl