Immunofluorescence staining of IDO1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human IDO1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.
IDO1 / IDO Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818064-100
Catalog Number: LS-C818064
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- Description
- IDO antibody LS-C818064 is an unconjugated rabbit polyclonal antibody to human IDO (IDO1). Validated for ELISA, ICC, IF, IHC and WB.
- Application
- ELISA, ICC, IF, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- IDO | Indole 2,3-dioxygenase | Indolamine 2,3 dioxygenase | IDO-1 | IDO1 | INDO | Indoleamine 2,3-dioxygenase | Indoleamine 2,3-dioxygenase 1
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P14902
- NCBI GenBank
- AAA36081.1 | NM_002164
- SwissProt
- P14902
Target
- Target
- Human IDO1 / IDO
- Antigen Species
- Human
- Epitope
- Human IDO
- Gene Name
- IDO1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human IDO/IDO1 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS