Anti-IDN3 Antibody - Western Blot. Western blot of Affinity Purified anti-IDN3 antibody shows detection of bands at ~315 kD and ~125 kD corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
IDN3 / NIPBL Antibody
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
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- Description
- NIPBL antibody LS-C60065 is an unconjugated goat polyclonal antibody to human NIPBL (IDN3). Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Dog, Mouse, Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDLS1 | CDLS | Delangin | IDN3 | NIPBL | Nipped-B homolog (Drosophila) | IDN3-B | Scc2 | SCC2 homolog | Nipped-B-like protein
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- Q6KC79
- NCBI GenBank
- NM_015384 | NP_056199.2
- SwissProt
- Q6KC79
Target
- Target
- Human IDN3 / NIPBL
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with this protein from human, rat, mouse, dog, bovine and orangutan based on 100% homology for the immunogen sequence. Expect reactivity with multiple splice variants of this protein. Cross reactivity with IDN3 homologues from other sources has not been determined.
- Gene Name
- NIPBL
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-365 of Human IDN3.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.6 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl