Western blot analysis of extracts from NIH/3T3 cells, treated with TNF-a (20ng/ml, 5mins), using CD50/ICAM-3 (Ab-518) antibody.
ICAM3 / CD50 Antibody (p518)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C822235-100
Catalog Number: LS-C822235
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- Description
- CD50 antibody LS-C822235 is an unconjugated rabbit polyclonal antibody to human CD50 (ICAM3) (p518). Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD50 | CDW50 | ICAM-R | ICAM3 | CD50 antigen | ICAM-3
- Recommended Dilution: ELISA
- 1:5000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P32942
- NCBI GenBank
- NM_002162 | NP_002153.2
- SwissProt
- P32942
Target
- Target
- Human ICAM3 / CD50
- Antigen Species
- Human
- Epitope
- CD50/ICAM-3 (Ab-518) Antibody detects endogenous levels of total CD50/ICAM-3 protein.
- Gene Name
- ICAM3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized non-phosphopeptide derived from human CD50/ICAM-3 around the phosphorylation site of serine 518 (S-G-SP-Y-H).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl