Detection of Human ICAM-3 by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-3 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
ICAM3 / CD50 Antibody (aa497-547)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C290976-10
Catalog Number: LS-C290976
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- Description
- CD50 antibody LS-C290976 is an unconjugated rabbit polyclonal antibody to human CD50 (ICAM3) (aa497-547). Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD50 | CDW50 | ICAM-R | ICAM3 | CD50 antigen | ICAM-3
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P32942
- NCBI GenBank
- NM_002162 | NP_002153.2
- SwissProt
- P32942
Target
- Target
- Human ICAM3 / CD50
- Antigen Species
- Human
- Epitope
- Region between residue 497 to 547 of human Intercellular Adhesion Molecule 3 using the numbering given in entry NP_002153.2 (GeneID 3385).
- Gene Name
- ICAM3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 3385 corresponding to amino acids between 497 and 547 of UniprotID: P32942
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate