Western blot analysis of extracts from Hela cells treated with TNF-a (20ng/ml, 5 mins), using ICAM-1 (phospho-Tyr512) antibody, Line 1 and 2).
ICAM-1 / CD54 Antibody (phospho-Tyr512)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826276-100
Catalog Number: LS-C826276
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- Description
- CD54 antibody LS-C826276 is an unconjugated rabbit polyclonal antibody to human CD54 (ICAM-1) (pTyr512). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BB2 | CD54 antigen | CD54 | ICAM-1 | ICAM1 | p3.58
- Recommended Dilution: ELISA
- 1:40000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P05362
- NCBI GenBank
- NM_000201 | NP_000192.2
- SwissProt
- P05362
Target
- Target
- Human ICAM-1 / CD54
- Antigen Species
- Human
- Epitope
- ICAM-1 (phospho-Tyr512) antibody detects endogenous levels of ICAM-1 only when phosphorylated at throsine 512. (Human: Tyr512)
- Gene Name
- ICAM1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human ICAM-1 around the phosphorylation site of tyrosine 512 (K-K-YP-R-L).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl