LSBio
ICAM-1 / CD54 Antibody (clone 84H10)

ICAM-1 / CD54 Antibody (clone 84H10)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation
Reactivity:Human
Format:Liquid
See all Human ICAM-1 / CD54 antibodies →
$486.00each
Catalog Number: LS-C45179-0.2

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Overview

Description
CD54 antibody LS-C45179 is an unconjugated mouse monoclonal antibody to human CD54 (ICAM-1). Validated for ELISA, Flow, IHC and IP.
Application
ELISA, FC, IHC, IHC-F, IP
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
BB2 | CD54 antigen | CD54 | ICAM-1 | ICAM1 | p3.58
Recommended Dilution: FC
1:1 - 1:10
Recommended Dilution: IHC-F
1:20 - 1:50
UniProt Number
P05362
SwissProt
P05362

Target

Target
Human ICAM-1 / CD54
Antigen Species
Human
Epitope
Recognizes the D1 domain of ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD. Clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. Removal of sodium azide is recommended prior to use in functional assays - XXX recommend the use of EQU003 for this purpose.
Gene Name
ICAM1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
84H10
Isotype
IgG1

Immunogen

Immunogen
K562 cell line.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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