Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysates showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.
HYOU1 / ORP150 Antibody (clone 6G7-2H5)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C775891-100
Catalog Number: LS-C775891
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- Description
- ORP150 antibody LS-C775891 is an unconjugated mouse monoclonal antibody to human ORP150 (HYOU1). Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- GRP-170 | Grp170 | Glucose-regulated protein 170 | Hypoxia up-regulated 1 | Hypoxia up-regulated protein 1 | HSP12A | ORP150 | HYOU1 | ORP-150
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9Y4L1
- NCBI GenBank
- NM_006389 | NP_006380.1
- SwissProt
- Q9Y4L1
Target
- Target
- Human HYOU1 / ORP150
- Antigen Species
- Human
- Epitope
- Detects ~170 kDa.
- Gene Name
- HYOU1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 6G7-2H5
- Isotype
- IgG2a
- Origin Species
- Human
Immunogen
- Immunogen
- Raised against a synthetic peptide of human GRP170
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS