Mouse HSP60 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. 2 uL anti-Mouse HSP60 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Mouse HSP60 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa.
HSPD1 / HSP60 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818558-100
Catalog Number: LS-C818558
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- Description
- HSP60 antibody LS-C818558 is an unconjugated rabbit polyclonal antibody to mouse HSP60 (HSPD1). Validated for IHC, IP and WB.
- Application
- IHC-P, IP, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSPD1 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
- Recommended Dilution: IHC-P
- 1:1000 - 1:4000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10809
- NCBI GenBank
- NM_002156 | NP_002147.2
- SwissProt
- P10809
Target
- Target
- Mouse HSPD1 / HSP60
- Antigen Species
- Mouse
- Epitope
- Mouse HSP60
- Gene Name
- HSPD1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse HSPD1 / HSP60 protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS