All lanes: Anti-Hsp60 Antibody at 1:16000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
HSPD1 / HSP60 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C757594-200
Catalog Number: LS-C757594
For bulk orders or custom formulations:
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- Description
- HSP60 antibody LS-C757594 is an unconjugated rabbit polyclonal antibody to HSP60 (HSPD1) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
- Application
- ELISA, FC, IF, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Predicted Reactivity
- Bovine, Chicken, Hamster
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSPD1 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
- Recommended Dilution: FC
- 1:25
- Recommended Dilution: IF/ICC
- 1:25
- Recommended Dilution: IHC-P
- 1:25
- Recommended Dilution: WB
- 1:16000
- UniProt Number
- P10809
- NCBI GenBank
- NM_002156 | NP_002147.2
- SwissProt
- P10809
Target
- Target
- Human HSPD1 / HSP60
- Antigen Species
- Human
- Gene Name
- HSPD1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This Hsp60 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 396-430 amino acids from human Hsp60.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 200 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS