Immunofluorescence staining of HSPB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human HSPB8 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.
HSPB8 / H11 / HSP22 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Cynomolgus Monkey, Human, Mouse, Rat |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C814875-100
Catalog Number: LS-C814875
For bulk orders or custom formulations:
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- Description
- HSP22 antibody LS-C814875 is an unconjugated rabbit polyclonal antibody to HSP22 (HSPB8 / H11) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC-P
- Reactivity
- Cyno, Hu, Ms, Rt
- Predicted Reactivity
- Bovine, Dog, Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alpha-crystallin C chain | CMT2L | Crystallin alpha c | DHMN2 | E2-induced gene 1 protein | CRYAC | E2IG1 | HMN2 | HSPB8 | H11 | Heat shock 27kDa protein 8 | Heat shock protein beta-8 | Heat shock 22kDa protein 8 | HMN2A | HSP22 | Protein kinase H11
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:100 - 1:500
- UniProt Number
- Q9UJY1
- NCBI GenBank
- NM_014365 | NP_055180.1
- SwissProt
- Q9UJY1
Target
- Target
- Human HSPB8 / H11 / HSP22
- Antigen Species
- Human
- Epitope
- Human HSPB8/Cryac
- Gene Name
- HSPB8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the center region of the Human HSPB8/Cryac
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS