LSBio
HSPB1 / HSP27 Antibody (phospho-Ser82)

HSPB1 / HSP27 Antibody (phospho-Ser82)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunoprecipitation, Western Blot
Reactivity:Bovine, Chicken, Dog, Guinea Pig, Human, Sheep
Format:Liquid
See all Human HSPB1 / HSP27 antibodies →
$554.00each
Catalog Number: LS-C15667-50

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Overview

Description
HSP27 antibody LS-C15667 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB1) (pSer82) from human. It is reactive with human, bovine, chicken and other species. Validated for IP and WB.
Application
IP, WB
Reactivity
Bov, Can, Ck, GP, Hu, Sh
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Recommended Dilution: IP
1:50
UniProt Number
P04792
SwissProt
P04792

Target

Target
Human HSPB1 / HSP27
Antigen Species
Human
Epitope
Detects an ~27kD protein, corresponding to the apparent molecular mass of phosphorylated Hsp27 (Ser82) on SDS-PAGE immunoblots. Species cross-reactivity: human, bovine, canine, chicken, guinea pig and sheep. This antibody is specific for phosphorylated Hsp27 (Ser82) and does not exhibit reactivity with non-phosphorylated Hsp27. Specificity for phospho-Hsp27 was demonstrated in peptide competition studies with phosphorylated and non-phosphorylated peptides.
Gene Name
HSPB1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
An 11 residue synthetic peptide, LSRQL[pS]SGVSE(C) corresponding to amino acids 77-87 of human Hsp27 with a cysteine (C) residue added and the peptide coupled to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Supplied as a liquid.
Volume
50 Microliter
Preservative
No
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.