Western blot analysis of extracts from HeLa cells using HSP27 (Ab-15) antibody (Line1 and 2) and HSP27 (phospho-Ser15) antibody (Line 3 and 4).
HSPB1 / HSP27 Antibody (phospho-Ser15)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C827167-100
Catalog Number: LS-C827167
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- Description
- HSP27 antibody LS-C827167 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB1) (pSer15) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
- UniProt Number
- P04792
- NCBI GenBank
- NM_001540 | NP_001531.1
- SwissProt
- P04792
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:20000 |
| IHC | 1:50 - 1:100 |
| WB | 1:500 - 1:1000 |
Target
- Target
- Human HSPB1 / HSP27
- Antigen Species
- Human
- Epitope
- HSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15.
- Gene Name
- HSPB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl