1:200 staining human kidney sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSPB1 / HSP27 Antibody (phospho-Ser15)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800942-100
Catalog Number: LS-C800942
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HSP27 antibody LS-C800942 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB1) (pSer15) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P04792
- NCBI GenBank
- NM_001540 | NP_001531.1
- SwissProt
- P04792
Target
- Target
- Human HSPB1 / HSP27
- Antigen Species
- Human
- Epitope
- Phospho-HSP27 (Ser15) Antibody detects endogenous levels of HSP27 only when phosphorylated at Serine 15.
- Gene Name
- HSPB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human HSP27 around the phosphorylation site of Serine 15.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS