HSPB1 / HSP27 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$624.00each
Catalog Number: LS-C15718-50
Catalog Number: LS-C15718
For bulk orders or custom formulations:
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- Description
- HSP27 antibody LS-C15718 is an unconjugated mouse monoclonal antibody to HSP27 (HSPB1) from human. It is reactive with human and monkey. Validated for ELISA, ICC, IF, IHC, IP and WB.
- Application
- ELISA, ICC, IF, IHC, IP, WB
- Reactivity
- Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
- Recommended Dilution: IHC
- 5 ug/ml
- UniProt Number
- P04792
- NCBI GenBank
- NM_001540|NP_001531.1
- SwissProt
- P04792
Target
- Target
- Human HSPB1 / HSP27
- Antigen Species
- Human
- Epitope
- Recognizes an ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins.
- Gene Name
- HSPB1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Purified Hsp24 isolated from MCF-7, a human breast tumor cell line (7). Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
- Preservative
- No
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS