Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody (M04), clone 3G3.Lane 1: HSPB1 transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
HSPB1 / HSP27 Antibody (clone 3G3)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$342.00each
Catalog Number: LS-C197469-100
Catalog Number: LS-C197469
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- Description
- HSP27 antibody LS-C197469 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, IF, IP and WB.
- Application
- ELISA, IF, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
- Recommended Dilution: IF/ICC
- 10 ug/ml
- UniProt Number
- P04792
- NCBI GenBank
- NM_001540 | NP_001531.1
- SwissProt
- P04792
Target
- Target
- Human HSPB1 / HSP27
- Antigen Species
- Human
- Epitope
- Human HSPB1 / HSP27
- Gene Name
- HSPB1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 3G3
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- HSPB1 (NP_001531, aa 96-205). A partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS