Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (SPC-747) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.
HSPA8 / HSC70 Antibody (AP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Protozoa / Protozoan Parasite |
| Format: | Liquid |
$484.00each
Catalog Number: LS-C821363-100
Catalog Number: LS-C821363
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- Description
- HSC70 antibody LS-C821363 is an AP-conjugated rabbit polyclonal antibody to trypanosoma brucei HSC70 (HSPA8). Validated for WB.
- Application
- WB
- Reactivity
- Proto
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Heat shock 70 kDa protein 8 | Heat shock 70kd protein 10 | Heat shock 70kD protein 8 | Heat shock cognate protein 54 | HSP71 | HSPA10 | HSC71 | HSP73 | HSPA8 | LPS-associated protein 1 | Heat shock 70kDa protein 8 | NIP71 | HSC54 | HSC70 | LAP1
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P11142
- NCBI GenBank
- NM_006597 | NP_006588.1
- SwissProt
- P11142
Target
- Target
- Trypanosoma brucei HSPA8 / HSC70
- Antigen Species
- Protozoa / Protozoan Parasite
- Epitope
- Detects ~71 kDa.
- Gene Name
- HSPA8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the mid-protein of Trypanosoma brucei brucei TbHsp70 (aa. 200-300)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Alkaline Phosphatase
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS