Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 94 kDa GRP94 protein using Mouse Anti-GRP94 Monoclonal Antibody, Clone 6B12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates (10 ug). Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GRP94 Monoclonal Antibody at 1:1000 for O/N at 4°C. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 94 kDa.
HSP90B1 / GP96 / GRP94 Antibody (clone 6B12)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C775164-100
Catalog Number: LS-C775164
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- Description
- GRP94 antibody LS-C775164 is an unconjugated mouse monoclonal antibody to GRP94 (HSP90B1 / GP96) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ECGP | Endoplasmin | gp96 homolog | gp96 | GRP-94 | GRP94 | Tumor rejection antigen 1 | TRA1 | HSP90B1
- UniProt Number
- P14625
- NCBI GenBank
- NM_003299 | NP_003290.1
- SwissProt
- P14625
Recommended Dilution
| Application | Dilution |
|---|---|
| WB | 1:1000 |
Target
- Target
- Human HSP90B1 / GP96 / GRP94
- Antigen Species
- Human
- Epitope
- Detects 94 kDa band corresponding to GRP94.
- Gene Name
- HSP90B1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 6B12
- Isotype
- IgG2a
- Origin Species
- Human
Immunogen
- Immunogen
- KDEL peptide (C-AVKDEL -COOH) conjugated at the N-Terminus to Bovine Serum Albumin (BSA)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS