affinity purified anti-Hsp90 acetyl K294 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
HSP90AA1 / Hsp90 Alpha A1 Antibody (K294)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745306-25
Catalog Number: LS-C745306
For bulk orders or custom formulations:
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- Description
- Hsp90 Alpha A1 antibody LS-C745306 is an unconjugated rabbit polyclonal antibody to Hsp90 Alpha A1 (HSP90AA1) (K294) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Predicted Reactivity
- Chicken, Fruit Fly, Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- EL52 | HSP86 | Hsp89 | HSP89A | HSPC1 | Heat shock 86 kDa | Hsp90 | HSP90A | HSP90AA1 | HSP90N | HSPCA | HSPCAL4 | HSPN | LAP2 | HSP 86 | HSPCAL1
- Recommended Dilution: ELISA
- 1:2500 - 1:10000
- Recommended Dilution: IHC
- 5 - 10 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:2500
- UniProt Number
- P07900
- NCBI GenBank
- NM_005348 | NP_005339.3
- SwissProt
- P07900
Target
- Target
- Human HSP90AA1 / Hsp90 Alpha A1
- Antigen Species
- Human
- Epitope
- Anti-Heat Shock Protein HSP 90-alpha acetyl specific K294 antibody is directed against human Hsp90 protein acetylated at K294. While ELISA data show strong reactivity with the acetylated form of the immunizing peptide and minimal reactivity with the non-acetylated form, to date western blotting data are not definitive for acetyl K294 specificity as blots show equivalent staining of lysates from cells either treated or untreated with Trichostatin A (an HDAC inhibitor). A BLAST analysis was used to suggest cross-reactivity with Hsp90 from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with Hsp90 from other species is unknown.
- Gene Name
- HSP90AA1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Heat Shock Protein HSP 90-alpha acetyl specific K294 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.96 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl