Western Blot analysis of Human HeLa showing detection of 90 kda Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone M10E3R. Lane 1: Molecular Weight Ladder (MW). Lane 2: Hela (10ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 90 kda. Other Band(s):.
HSP90AA / HSP90 Alpha Antibody (clone M10E3R, APC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Recombinant Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$504.00each
Catalog Number: LS-C775146-100
Catalog Number: LS-C775146
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- Description
- HSP90 Alpha antibody LS-C775146 is an APC-conjugated mouse recombinant monoclonal antibody to human HSP90 Alpha (HSP90AA). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Recombinant Antibodies
- Recommended Dilution: WB
- 1:1000
Target
- Target
- Human HSP90AA / HSP90 Alpha
- Antigen Species
- Human
- Epitope
- Detects 90 kDa. Does not detect HSP90 beta.
Antibody
- Host Species
- Mouse
- Clonality
- Recombinant Monoclonal
- Clone
- M10E3R
- Isotype
- IgG2a
Immunogen
- Immunogen
- Purified HSP90
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Allophycocyanin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS