Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of rabbit anti-HSF1 antibody. Lane 1: Ladder (Opal PreStained p/n MB-210-0500). Lane 2: A431 Whole Cell lysate p/n W09-000-361. Load: 35 µg per lane. Primary antibody: HSF1 antibody at 1.0 ug/ml for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Block: Univeral Buffer BlockOut (MB-073) for 30 min at room temperature. Predicted/Observed size: ~57kDa for HSF1 in A431 whole cell lysate.
HSF1 Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745411-25
Catalog Number: LS-C745411
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HSF1 antibody LS-C745411 is an unconjugated rabbit polyclonal antibody to human HSF1 (N-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HSF1 | HSTF 1 | Heat shock factor protein 1 | HSF 1 | HSTF1
- UniProt Number
- Q00613
- NCBI GenBank
- NM_005526 | NP_005517.1
- SwissProt
- Q00613
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:50000 |
| WB | 1 µg/ml |
Target
- Target
- Human HSF1
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with this protein in mouse and bovine species based on 100% homology for the immunogen sequence.
- Gene Name
- HSF1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Affinity purified Anti-HSF1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the n-terminal portion of human HSF1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.96 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
