Immunofluorescence staining of PC3 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HSD-1 / SPAG8 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C673990-50
Catalog Number: LS-C673990
For bulk orders or custom formulations:
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- Description
- SPAG8 antibody LS-C673990 is an unconjugated rabbit polyclonal antibody to human SPAG8 (HSD-1). Validated for ELISA and IF.
- Application
- ELISA, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Sperm membrane protein BS-84 | Sperm-associated antigen 8 | SMP-1 | Sperm associated antigen 8 | SMP1 | SPAG3 | BS-84 | HSD-1 | HSMP-1 | SPAG8 | Sperm membrane protein 1
- UniProt Number
- Q99932
- NCBI GenBank
- AAH19247.1 | BC019247
- SwissProt
- Q99932
Target
- Target
- Human HSD-1 / SPAG8
- Antigen Species
- Human
- Gene Name
- SPAG8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Sperm-associated antigen 8 protein (236-409AA)
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS